Antigen

Antigen Class

TFs and others

Antigen

ATF4

Cell type

Cell type Class

Blood

Cell type

SUP-B15

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

SUPB15 (ALL cell line)

cell type

ALL

chip antibody

ATF4 Rabbit mAb (Cell singal #11815)

treatment

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked, lysed, and sonicated according to the ENCODE project ChIPseq guidelines (https://www.encodeproject.org/about/experiment-guidelines/). 5ug of ATF4 antibody was used for IP Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

76208902

Reads aligned (%)

98.8

Duplicates removed (%)

14.2

Number of peaks

2949 (qval < 1E-05)

hg19

Number of total reads

76208902

Reads aligned (%)

98.3

Duplicates removed (%)

15.7

Number of peaks

2622 (qval < 1E-05)