Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ATF4

Cell type

Cell type Class
Blood
Cell type
SUP-B15
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
SUPB15 (ALL cell line)
cell type
ALL
chip antibody
ATF4 Rabbit mAb (Cell singal #11815)
treatment
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked, lysed, and sonicated according to the ENCODE project ChIPseq guidelines (https://www.encodeproject.org/about/experiment-guidelines/). 5ug of ATF4 antibody was used for IP Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
76208902
Reads aligned (%)
98.8
Duplicates removed (%)
14.2
Number of peaks
2949 (qval < 1E-05)

hg19

Number of total reads
76208902
Reads aligned (%)
98.3
Duplicates removed (%)
15.7
Number of peaks
2622 (qval < 1E-05)

Base call quality data from DBCLS SRA